Enzyme-linked Immunosorbent Assay:Article Title: Multispecific antibodies, multispecific activatable antibodies and methods of using the same
Article Snippet: .. Human Jagged 1-Fc (R&D Systems; Cat #1277-JG-050), human EGFR (R&D Systems, Cat #344-ER-050) or human CTLA4 (R&D Systems; Cat #325-CT-200/CF) were adsorbed onto wells of a 96-well ELISA plate. .. Human Jagged 1-Fc (R&D Systems; Cat #1277-JG-050), human EGFR (R&D Systems, Cat #344-ER-050) or human CTLA4 (R&D Systems; Cat #325-CT-200/CF) were adsorbed onto wells of a 96-well ELISA plate.
Article Title: Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis
Article Snippet: Absorbance was measured at 450 nm in a Spectra Max M5 microplate reader (Molecular Devices). .. Affinity of GLV-1h376-encoded CTLA4 scAb to human CTLA4 and lack of cross-reactivity to mouse CTLA4 in vitro was tested using the same ELISA setup, but 96-well plates were pre-coated with either rh CTLA4 Fc chimera or rm CTLA4 Fc chimera, respectively, both from R&D Systems, and incubated with dilutions of purified CTLA4 scAb as test samples. .. Timed-pregnant NOD.Cg-Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice were purchased from The Jackson Laboratory and allowed to acclimatize for 3–6 days before giving birth under pathogen-free conditions in HEPA-filtered, individually ventilated disposable cages (Innovive) with irradiated food and pre-bottled acidified water (Innovive) in the animal facilities of Explora Biolabs at the San Diego Science Center.
Article Title: Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis.
Article Snippet: Absorbance was measured at 450 nm in a Spectra Max M5 microplate reader (Molecular Devices). .. Affinity of GLV1h376-encoded CTLA4 scAb to human CTLA4 and lack of crossreactivity to mouse CTLA4 in vitro was tested using the same ELISA setup, but 96-well plates were pre-coated with either rh CTLA4 Fc chimera or rm CTLA4 Fc chimera, respectively, both from R&D Systems, and incubated with dilutions of purified CTLA4 scAb as test samples. .. Timed-pregnant NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice were purchased from The Jackson Laboratory and allowed to acclimatize for 3–6 days before giving birth under pathogen-free conditions in HEPA-filtered, individually ventilated disposable cages (Innovive) with irradiated food and pre-bottled acidified water (Innovive) in the animal facilities of Explora Biolabs at the San Diego Science Center.
In Vitro:Article Title: Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis
Article Snippet: Absorbance was measured at 450 nm in a Spectra Max M5 microplate reader (Molecular Devices). .. Affinity of GLV-1h376-encoded CTLA4 scAb to human CTLA4 and lack of cross-reactivity to mouse CTLA4 in vitro was tested using the same ELISA setup, but 96-well plates were pre-coated with either rh CTLA4 Fc chimera or rm CTLA4 Fc chimera, respectively, both from R&D Systems, and incubated with dilutions of purified CTLA4 scAb as test samples. .. Timed-pregnant NOD.Cg-Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice were purchased from The Jackson Laboratory and allowed to acclimatize for 3–6 days before giving birth under pathogen-free conditions in HEPA-filtered, individually ventilated disposable cages (Innovive) with irradiated food and pre-bottled acidified water (Innovive) in the animal facilities of Explora Biolabs at the San Diego Science Center.
Article Title: Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis.
Article Snippet: Absorbance was measured at 450 nm in a Spectra Max M5 microplate reader (Molecular Devices). .. Affinity of GLV1h376-encoded CTLA4 scAb to human CTLA4 and lack of crossreactivity to mouse CTLA4 in vitro was tested using the same ELISA setup, but 96-well plates were pre-coated with either rh CTLA4 Fc chimera or rm CTLA4 Fc chimera, respectively, both from R&D Systems, and incubated with dilutions of purified CTLA4 scAb as test samples. .. Timed-pregnant NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice were purchased from The Jackson Laboratory and allowed to acclimatize for 3–6 days before giving birth under pathogen-free conditions in HEPA-filtered, individually ventilated disposable cages (Innovive) with irradiated food and pre-bottled acidified water (Innovive) in the animal facilities of Explora Biolabs at the San Diego Science Center.
Incubation:Article Title: Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis
Article Snippet: Absorbance was measured at 450 nm in a Spectra Max M5 microplate reader (Molecular Devices). .. Affinity of GLV-1h376-encoded CTLA4 scAb to human CTLA4 and lack of cross-reactivity to mouse CTLA4 in vitro was tested using the same ELISA setup, but 96-well plates were pre-coated with either rh CTLA4 Fc chimera or rm CTLA4 Fc chimera, respectively, both from R&D Systems, and incubated with dilutions of purified CTLA4 scAb as test samples. .. Timed-pregnant NOD.Cg-Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice were purchased from The Jackson Laboratory and allowed to acclimatize for 3–6 days before giving birth under pathogen-free conditions in HEPA-filtered, individually ventilated disposable cages (Innovive) with irradiated food and pre-bottled acidified water (Innovive) in the animal facilities of Explora Biolabs at the San Diego Science Center.
Article Title: Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis.
Article Snippet: Absorbance was measured at 450 nm in a Spectra Max M5 microplate reader (Molecular Devices). .. Affinity of GLV1h376-encoded CTLA4 scAb to human CTLA4 and lack of crossreactivity to mouse CTLA4 in vitro was tested using the same ELISA setup, but 96-well plates were pre-coated with either rh CTLA4 Fc chimera or rm CTLA4 Fc chimera, respectively, both from R&D Systems, and incubated with dilutions of purified CTLA4 scAb as test samples. .. Timed-pregnant NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice were purchased from The Jackson Laboratory and allowed to acclimatize for 3–6 days before giving birth under pathogen-free conditions in HEPA-filtered, individually ventilated disposable cages (Innovive) with irradiated food and pre-bottled acidified water (Innovive) in the animal facilities of Explora Biolabs at the San Diego Science Center.
Purification:Article Title: Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis
Article Snippet: Absorbance was measured at 450 nm in a Spectra Max M5 microplate reader (Molecular Devices). .. Affinity of GLV-1h376-encoded CTLA4 scAb to human CTLA4 and lack of cross-reactivity to mouse CTLA4 in vitro was tested using the same ELISA setup, but 96-well plates were pre-coated with either rh CTLA4 Fc chimera or rm CTLA4 Fc chimera, respectively, both from R&D Systems, and incubated with dilutions of purified CTLA4 scAb as test samples. .. Timed-pregnant NOD.Cg-Prkdc scid Il2rg tm1Wjl /SzJ (NSG) mice were purchased from The Jackson Laboratory and allowed to acclimatize for 3–6 days before giving birth under pathogen-free conditions in HEPA-filtered, individually ventilated disposable cages (Innovive) with irradiated food and pre-bottled acidified water (Innovive) in the animal facilities of Explora Biolabs at the San Diego Science Center.
Article Title: Humanized Mice with Subcutaneous Human Solid Tumors for Immune Response Analysis of Vaccinia Virus-Mediated Oncolysis.
Article Snippet: Absorbance was measured at 450 nm in a Spectra Max M5 microplate reader (Molecular Devices). .. Affinity of GLV1h376-encoded CTLA4 scAb to human CTLA4 and lack of crossreactivity to mouse CTLA4 in vitro was tested using the same ELISA setup, but 96-well plates were pre-coated with either rh CTLA4 Fc chimera or rm CTLA4 Fc chimera, respectively, both from R&D Systems, and incubated with dilutions of purified CTLA4 scAb as test samples. .. Timed-pregnant NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice were purchased from The Jackson Laboratory and allowed to acclimatize for 3–6 days before giving birth under pathogen-free conditions in HEPA-filtered, individually ventilated disposable cages (Innovive) with irradiated food and pre-bottled acidified water (Innovive) in the animal facilities of Explora Biolabs at the San Diego Science Center.
Recombinant:Article Title: Humanised anti CTLA-4 antibodies
Article Snippet: .. Recombinant CTLA4-fusion protein comprising the extracellular domain of human CTLA4 fused to the human IgG1 constant domain was purchased from R&D Systems (Oxford, UK). .. Extracellular CTLA4 fragment was prepared by proteolytic cleavage of the CTLA4-Fc fusion protein with Factor Xa (Qiagen, Crawley, UK) followed by subsequent removal of the protease using Factor Xa removal resin (Qiagen) and of the cleaved Fc fragment using Protein A-agarose to leave the CTLA4 extracellular domain only.
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